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BAX System PCR Assay for E. coli O157-H7 MP

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BAX System PCR Assay for E. coli O157-H7 MP PCR Pathogen Tests Hygiena

Hygiena | BAX® System PCR Assay for E. coli O157-H7 MP
This multiplex (MP) assay includes several targets on the E. coli O157:H7 genome for greater assurance that new, unusual or modified varieties of this complex organism are detected.

  • Automated DNA-based analysis instead of subjective plate counts
  • Specificity ≥ 99%
  • Reliably detects 104 cfu/mL in enriched samples
  • Tableted PCR reagents reduce operator error
  • Closed-cap system avoids amplicon contamination in the lab
  • Designed for efficient workflow and ease of use
  • PCR processing completes in 3.5 hours with the standard MP protocol (or about 2.5 hours with MP Express for beef)
  • Target: E. coli O157:H7
  • Matrices: Raw ground beef, beef trim, produce
  • Enrichment time: 8-24 hours, depending on food type, sample volume and enrichment media
  • PCR time: 3.5 hours
  • Detection level: 10^4 cfu/mL, after enrichment
  • Validations:
    • AOAC Research Institute - Performance Tested Method #050501 - validated on raw ground beef, beef trim, spinach and lettuce
    • AFNOR - QUA-18/04-03/08 - validated on raw beef, raw milk, fruits, vegetables, ready-to-eat meats, raw pork, raw horse, raw chicken
    • Health Canada - MFLP#30 - approved for ground beef, beef trim, dairy, meat, fruit, vegetables, dry foods, environmentals, animal feed
Catalog No.
Legacy Order Code Hygiena Product Code Description Quantity
D12404903 KIT2004 BAX® System Assay for E. coli O157:H7  96 tests per kit
Safety Data Sheets (SDS)
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